Quick Answer: When Working With Biological Agents, Secondary Containment Refers To The Protection Of:?

Secondary containment, which is the protection of the environment external to the laboratory from exposure to infectious materials, is provided by a combination of facility design and operational practices. To review, the three elements of containment include: Laboratory practice and technique. Safety equipment.

What is secondary containment in biosafety?

Secondary containment is the protection of the environment external to the laboratory from exposure to infectious materials and is provided by a combination of facility design and operational practices.

What is secondary containment in microbiology?

There are two levels of biological containment: primary and secondary. Primary containment protects people and the immediate laboratory environment from exposure to infectious agents. Secondary containment protects the environment external to the laboratory from exposure to infectious materials.

What is the biological containment?

Biological containment. (Science: molecular biology) refers to any number of methods to contain genetically engineered organisms by creating biochemical barriers to prevent them from growing outside the laboratory.

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Which containment is used to prevent the escape of microbes?

The biological safety cabinet is the principal device used to provide containment of infectious aerosols generated by many microbiological procedures.

What is the intention of secondary containment?

The purpose of secondary containment is to prevent releases of chemicals to the environment and reduce contamination of soil and groundwater. Secondary containment requirements provide temporary containment of accidental spills or leaks until appropriate response actions are taken by the owner/operator.

How does a Class 2 biological safety cabinet work?

Class II BSCs are designed with an open front with inward airflow (personnel protection), downward HEPA-filtered laminar airflow (product protection) and HEPA-filtered exhaust air (environmental protection). These cabinets are further differentiated by types based on construction, airflow and exhaust systems.

What is primary and secondary containment?

It confines the substance and, when all goes well, does not allow it to spill or leak outside of the container. Secondary containment, on the other hand, is intended to contain a spill or leak when the primary containment fails.

How does a biological safety cabinet work?

Like a chemical fume hood, a biosafety cabinet protects the user from hazardous material using directional air flow. Biosafety cabinets differ in that the air is also HEPA filtered, which removes biological contaminants. It uses a curtain of air and HEPA filters to provide both containment and a sterile environment.

What is the working principle of biological safety cabinet?

Class 2 Cabinets Used when working with low to moderate risk biological agents.

What is containment in safety?

Containment is the term used to describe methods, practices, procedures, facilities, and equipment used to safely manage biohazardous materials in the laboratory. The purpose of containment is to reduce or eliminate exposure to people or the environment to potentially hazardous agents.

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What is level of biological containment?

A biosafety level (BSL), or pathogen/protection level, is a set of biocontainment precautions required to isolate dangerous biological agents in an enclosed laboratory facility. The levels of containment range from the lowest biosafety level 1 (BSL-1) to the highest at level 4 (BSL-4).

What are the types of containment?


  • Introduction.
  • Containment Systems and their Function.
  • Types of containment.
  • Dual dry containment.
  • BWR Pressure suppression containment.
  • Ice condenser containment.
  • Negative pressure containment.
  • Passive containment.

What are containment facilities?

Containment means to restrict a new organism in a secure location or facility. Containment facilities are designed and operated to prevent the release of the organism – or its heritable material – into the wider environment.

What is primary containment?

A tank, vessel, pipe, transport vessel or equipment intended to serve as the primary container for, or used for the transfer of, a material. Primary containers may be designed with secondary containment systems to contain or control a release from the primary containment.

What is primary containment device?

Primary containment device: A contained workspace designed to provide protection to its operator, the laboratory environment and/or the work materials for activities where there is an aerosol hazard.

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